Bacteriology 221923. Syllabus. IInd Semester DMLT. HSBTE.

• 1.1 General characteristics of bacteria-morphology, staining, culture, biochemical

• 1.2 Characteristics, antibiotics related to Gram-Positive bacteria and their distribution:

• Gram Positive

• 1.3 Staphylococci

• 1.4 Strep to cocci and pneumococci

• 1.5 Enter obacteriacae-(Ecoli,Salmonella,Shigella

SECTION A

• 1. Which of the following is not a subfield of microbiology?

i) Bacteriology ii) Botany iii) Clinical Microbiology iv) Virology

• 2. The study of microbes is known as........

i) Bacteriology ii) Immunology iii) Haematology iv) Microbiology

• 3. Bacteria have...............

• i) DNA ii) RNA iii) Both DNA & RNA iv) None of these

• 4.  ........... Protect the cell from unwanted bacteria and adverse environmental conditions

• i) Pilli ii) Spores iii) Cytoplasm iv) None of these

• 6. Study of bacteria is known as

• a) Bacteriology b) Microbiology c) Immunology d) Serology

• 7. Bacteria are _________

• a) Prokaryotes b) Eukaryotes c) Both DNA & RNA d) None of these

• 8. _________ play an important role during cell division.

• a) Mesosome b) Pilli c) spores d) Cytoplasm

• 9. Streptococci are _________ bacteria.

• a) Gram+ b) Gram c) Both A & B d) None of these

• 10. Bacteriology is a branch of ________?

• a) Hematology b) Botany c) Microbiology d) Virology

• 11. Bacteria are microscopic _____ organisms.

• a) Unicellular b) Multicellular c) Both A & B d) None of these

• 12. The optimum temperature for growth of bacteria is_____.

• a) 40C b) 37C c) 60C d) 45C

• 13. A group of coccus popularly called______.

• a) Cocci b) Spores c) Cytoplasm d) None of these

• 14. Gram stain and acid-fast stain are the examples of_________

• a) simple staining b) Negative staining c) Differential staining d) Special

• 15. _____ protects the cells against harsh and adverse environment conditions.

• a) Spores b) Pilli c) Mesosomes d) Ribosomes

• 16. E-coli is ______ bacterial.

• a) Gram+ b) Gram c)Both A & B d) None of these

• 17. India ink preparation is also called_______.

• a) capsules Staining b) Spores staining c) Nucleus staining d) None of these

• 18. Temp for staphylococcus bacterial growth

• a) 0 degrees C-15 degrees C b) 10 degrees C-15 degrees C.

• b) c) 30 degrees C-37 degrees C d) All

• 19. Gram stain shows gram+ve bacteria__________.

• a) Red b) Blue c) Capsule d) Spores

• 20. Which bacteria are found in the large intestine of human beings?

• a) Streptococcus b) Salmonella c) Both A & B d) None of these

SECTION B

• 1. Salmonella gives citrate utilization test ______.

• 2. Give an example of gram-negative bacteria.

• 3. _______ test is commonly used for diagnosis of Salmonella Typhi.

• 4. Normal habitat of Streptococci is _______.

• 5. Define colony.

• 6. What are the biochemical tests for E.COLI?

• 7. What is Culture Media?

• 8. Name the possible pathogen that can invade the skin and cause tissue damage in humans

• 9. Staphylococci is a _________bacteria.

• 10. ________ are flame-shaped diplococci.

• 11. 5 Staphylococci produces _______ hemolysis on blood agar media.

• 12. Bacillary dysentery is caused by---------.

• 13. Define microbe

• 14. Write two causative agents of Gastrointestinal infection.

• 15. Staphylococci is a ____________ bacteria.

• 16. Flagella are organs of ______________.

• 17. ______ is a person who harbors a pathogenic organism.

• 18. Gram-negative cell wall is _________than a Gram positive.

• 19. Salmonella gives citrate utilization test positive. (T/F)

• 20. Flagella and pili are made of _________.

• 21. Cilia, fimbriae, and pili are all examples of structures used by microbes for adhesion. (True/False)

SECTION C

• 1. Write down the morphology of staphylococcus.

• 2. Write the biochemical characteristics of salmonella.

• 3. Write the morphological characteristics of streptococci

• 4. Write the laboratory diagnosis of shigella.

• 5. Explain the cultural characteristics of Streptococci.

• 6. Write a short note on the morphological characteristics of bacteria.

• 7. Describe the disease caused by E-coil.

• 8. Write a short note on different staining procedures. or

• 9. Write a note on various staining methods of bacteria.

• 10. Give the morphology and gram character of streptococci.

• 11. Give the lab diagnosis of UTI infections

• 12. Write the general characteristics of bacteria.

• 13. Write the Laboratory diagnosis of Shigella.

• 14. Write the morphology & staining of Staphylococci.(imp)

• 15. Write the cultural characteristics of pneumococci(Imp)

• 16. Write the Laboratory diagnosis of salmonella.

• 17. Write the morphology & staining of streptococci. (imp)

• 18. Explain the significance of different samples in Salmonella identification.

• 19. Draw the structure of Staphylococci and write its staining characteristics.

• 20. Write down the morphological characteristics of bacteria.

• 21. Describe the cultural characteristics of E-coil.

SECTION D

• 1. Describe morphology, Staining procedure, and cultural characteristics of streptococci

• 2. Explain the morphology and culture characteristics of Pneumococci

• 3. Give the morphology, staining, and culture characteristics of E.coli.

• 4. Describe the morphology, staining, and culture characteristics of shigella.

• 5. Give the morphology and staining. and biochemical characteristics of Staphylococci

• 6. Draw a labeled diagram of bacteria & explain the importance of Staining & cultural characteristics.

• 7. Write the characteristics and distribution of the bacteria Enterobacter family. Also, draw their diagrams.

UNIT II

SECTION A

Note: Multiple choice questions. All questions are compulsory (6x1=6)

• 1. Syphillis is caused by___________.

• a) T. Palladium b) Salmonella c) Proteus d) None of these

• 2. AFB is _________

• a) Acid Fast Bacteria b) Alkaline Fast Bacilli c) Arsenic Fast Bacteria d) Alcohol Fast Bacilli

• 3. Gram stain shows gram+ve bacteria__________.

• a) Red b) Blue c) Capsule d) Spores

• 4. Vibrio is __________.

• a) Rod b) Round c) Curve d) Comma

• 5. Which of the following microbe can survive only inside the cell(obligate intracellular pathogen)?

• a) Salmonella b) Mycobacterium c) Rickettsia d) Vibrio

• 6. Gram stain and acid-fast stain are the examples of _________

• a) simple staining b) Negative staining c) Differential staining d) Special staining

• 7. Which of the following bacteria are gram (-) and tend to colonize in moist areas?

• i) E.coli ii) Klebsiella iii) Mycobacteria iv) Pseudomonas

• 8. All of the following are gram-negative bacilli except

• i) Proteus spp ii) Pseudomonas spp iii) Neisseria spp iv) Klebsiella spp

SECTION B

Note: Objective/ Completion type questions. All questions are compulsory. (6x1=6)

• 1. Draw the structure of vibrio cholera.

• 2. Expand VDRL.

• 3. T. Pallidium cause the __________disease.

• 4. Name two AFB Microorganisms.

• 5. Expand STD?

• 6. ______is caused by Treponima Palidum?

• 7. Vibrio are ________ shaped. (Imp)

• 8. Flagella are organs of _________.

• 9. D r a w t h e s h a p e o f M y c o b a c t e r i u m Tuberculosis.

• 10. VDRL test is used to diagnose_________.

• 11. Leprosy is caused by _______.

• 12. Cholera is caused by _______.

• 13. N. gonorrhoea causes-----------.

• 14. The basic stain used in AFB staining is---------

• 15. Write one characteristic of Neisseria

• 16. Give two examples of gram-negative bacteria.

SEC TION C

Note: Short answer type questions. Attempt any eight questions out of ten questions. (8x4=32)

1. Write a note on Cholera red infection.

2. Differentiate Gram-positive and Gram-negative bacteria.

3. Write steps for staining of mycobacterium tuberculosis

4. What are the characteristics of Mycobacterium tuberculosis?

5. Explain the cultural characteristics of Vibrio cholerae.

6. Explain the staining of Vibrio Cholera.

7. What is Mycobacterium? How they differ from others.

8. What are mycobacteria? How do they differ from bacteria?

9. Write about the staining procedure to be adopted for Vibrio cholerae.

10. Write a short note on pseudomonas.

11. Give the test for M. Tuberculosis.

12. Explain the cultural characteristics of T. pallidium.

13. Explain the staining of pseudomonas

14. Write a short note on pseudomonas

SECTION D

Long answer type questions. Attempt any two questions out of three questions. (2x8=16)

• 1. Explain morphology, biochemical test, and lab diagnosis of Mycobacterium tuberculosis bacteria

• 2. Give the Lab diagnosis of M. Tuberculosis in detail.

• 3. Explain the morphology, staining, culture & biochemical characteristics of Mycobacterium Tuberculosis (V Important)

• 4. Describe the morphology, staining, pathogenic species, culture, and biochemical characteristics for the diagnosis of Neisseria.

• 5. Write a note on morphology staining and biochemical characteristics of Treponema Pallidum.

• 6. Write the Morphological characters, staining procedure biochemical characteristics, and distribution of vibrio cholerae.

Unit III

Bacterial pathogenicity

3.1 Introduction, pathogenicity & infection.

3.2 Sources of infection

3.3 Mode of spread of infection

3.4 Types of Infection

SECTION A

Note: Multiple choice questions. All questions are compulsory (6x1=6)

SECTION B

Note: Objective/ Completion type questions. All questions are compulsory. (6x1=6)

• 1. Define Virulence.

• 2. Define pathogenicity?

• 3. What are communicable diseases?

• 4. The infection that spreads by coughing, sneezing laughing, etc is called _______.

• 5. __________ is a person who harbors a pathogenic organism.

• 6. Define Iatrogenic infection.

• 7. HAI stands for ________.

• 8. The infection that spreads by coughing, sneezing, laughing etc is called ______

• 9. Write any two preventive measures to control bacterial infections.

• 10. Write the name of two communicable infections.

• 11. What are vectors?

• 12. Define Resistance

SECTION C

Note: Short answer type questions. Attempt any eight questions out of ten questions. (8x4=32)

• 1. Define infection and how it spreads in vivo.

• 2. Write the different sources of injection. OR Enlist the different sources of infection.

• 3. Write a note on the pathogenicity of bacteria.

• 4. Explain the classification of Infection.

• 5. Enlist the various preventions of infection.

• 6. Write a short note on airborne infection. How they can be controlled.

• 7. Describe different modes of transmission of infection.

• 8. Explain the common types of infection..

• 9. Explain the classification of Infection.

SECTION D

Long answer type questions. Attempt any two questions out of three questions. (2x8=16)

• 1. Explain about the bacterial pathogenicity?

• 2. Explain the various modes of spread of infection.

• 3. Enlist the different sources of infection along with their mode of transmission.

• 4. Explain pathogen, pathogenicity, and mechanism of microbial pathogenicity.

• 5. Write down any four preventions of infection. Explain types of infection.

Unit IV

Introduction

Common types and sources of nosocomial infection

Control of nosocomial infections

Section A

• 1. ___________ is the most commonly acquired hospital infection.

• a) Surgical wound infection b) Urinary tract infection

• c) Respiratory tract infection d) Infections diarrhea

• 2. Hospital-acquired infection is also known as :

• a) Nasocomial infection b) Nosocomial infection

• c) Nosocongenial infection d) Nosocomial infection

• 3. Which one is a Hospital-acquired infection?

• a) HIV b) Nosocomial infection c) both A & B d) None of these

• 4. Factors influencing the nosocomial infection are

• a) Patient susceptibility b) Microbial agents c) Environment Factors d) All of the above

• 5. Nosocomial infection is also known as______.

• a) Hospital-acquired infection b) Home-acquired infection

• c) Acquired infection d) None of these

• 6. ........... is the most commonly acquired hospital infection.

• i) Surgical wound infection ii) Urinary tract infection

• iii) Respiratory tract infection iv) Infectious diarrhea

  SEC B

• 1. ______ and ______ are the most common causes of infection in hospitals

• 2. Write down the classification of nosocomial infection.

• 3. Name any two sources of nosocomial infection with example(IMP)...

• 4. What is nosocomial infection(IMP).

• 5. Antibiotic-resistant Gastrointestinal organisms that transmit the infection through the hospital are called ______.

SEC C

• 1. Write the control measures for nosocomial infection

• 2. Write the common types of Nosocomial infection. Write in brief about the control of nosocomial infections.

• 3. Explain the various sources of nosocomial Infection.

• 4. Write a short note on nosocomial infection.

• 5. Write the control measures of nosocomial infection(IMP).

• 6. Explain the common types of Nosocomial infection.

• 7. Write about various measures for prevention of nosocomial infection.

SEC D

• 1. What are Nosocomial infections? Discuss its different types.

• 2. Enlist the different sources of infection along with their mode of transmission

• 3. Explain the factors that influence the development of the nosocomial infection.

• 4. Write the various classifications of infection.

• 5. Explain Nosocomial infection and its types of infection in detail.

• 6. Describe the different types of sources of nosocomial infection in detail.

UNIT V

Laboratory diagnosis of infectious diseases

5.1 Septicemia and bacteremia

5.2 Respiratory tract infections (Throat Swab and Sputum sample)

5.3 Wound infections

5.4 Urinary tract infections

5.5 Enteric fever

5.6 Intestinal infection

5.7 Meningitis

SEC A

1. __________cause intestinal infection.

a) Virus b) Parasite c) Capsule d) Spores

2. UTI stands for _______

a) Upper tract infection  b) Urinary tract infection

c) Urine infection   d) None of these

3. _______ is the common normal flora of the upper respiratory tract

a) Lactobacillus spp b) Staphylococcus spp

c) Vibrio spp   d) None of the above

4. UTI stands for _________

a) Upper tract infection b) Urinary tract infection c) Urine infection d) None of these

SEC B

1. Respiratory infection is mainly caused by_________.

2. Name the bacteria that cause intestinal infection

3. Respiratory infection is mainly caused by ____.

4. Define Septicemia.

5. Wound infections are mainly transmitted by ______ and ________.

6. RTI stands for_________.

7. _______group of bacteria is responsible for Enteric fever.

   SEC C

8. Write about the collection and processing of throat swabs.

9. What are intestinal infections? Mention at least two causative reasons.

10. Write the Laboratory diagnosis of Intestinal infection

11. Write the Laboratory diagnosis of Intestinal Infection.

12. Write a note on wound infection

13. Write two causative agents of Gastrointestinal infection

14. Write a short note on Urinary tract infection.

15. Write the Laboratory diagnosis of Respiratory tract Infection.

16. How the Laboratory Diagnosis of Respiratory Tract Infection is carried out?

17. Give the lab diagnosis of UTI infections. characteristics of Pneumococci.

18. Give the lab diagnosis of UTI infections.

19. Give the lab diagnosis of wound infections.

20. Write about lab diagnosis of wound infection (IMP)

21. What is Enteric fever? How IS it diagnosed?

22. Describe the processing of sputum samples.

23. Write the Laboratory diagnosis of Intestinal infection.

24. Write the lab diagnosis of respiratory tract infection.

25. Write about the collection and processing of sputum samples.

26. What are the symptoms of intestinal infection?

27. What are the causes of intestinal infection?

28. Write a note on typhoid fever.

29. How to collect urine samples.

30. Explain the significance of different samples in RTI

SEC D

1. Describe the Laboratory diagnosis of Respiratory Tract infections(IMP).

2. Explain lab diagnosis of Respiratory tract infection.

3. Explain lab diagnosis of UTI infection in detail.

4. Write the characteristics, distribution, and lab diagnosis of E-coli or explain the lab diagnosis of RTI in detail.

5. Mention about lab diagnosis procedure of enteric fever.

This is all about the Important questions in the subject Bacteriology IInd Semester DMLT. Stay tuned with alokpdf.com for more updates

Dr Pramila Singh